REPUBLIC OF KOREA, RURAL DEVELOPMENT ADMINISTRATION
发明人:
HA SUN HWA,KIM JU KON,JUNG HA RIN,KIM JUNG BONG,KIM YOUNG MI,SUH SEOK-CHEOL,SHI LIANG YING,KWEON SOON JONG,KIM DONG HERN
申请号:
US20080675240
公开号:
US2010299783(A1)
申请日:
2008.08.29
申请国别(地区):
美国
年份:
2010
代理人:
摘要:
The present invention relates to fusion polynucleotides for biosynthesis of beta-carotene and a method for producing beta-carotene using the same. More particularly, it relates to fusion polynucleotides encoding phytoene synthase, connective sequences of FMDV-derived 2A sequence or internal ribosome entry site (IRES) and carotene desaturase, and a method for producing beta-carotenes using the same. Fusion polynucleotides and the recombinant vector using the same of the present invention have the effect of expression both phytoene synthase gene and carotene desaturase gene stably within cell transformants. Accordingly, fusion polynucleotides of the present invention can be used to regulate the biosynthetic metabolism of plant producing beta-carotene. Furthermore, it can be applied to effectively increase the content of beta-carotene, a useful metabolite.