A preparation method of chitosan fiber matrix is disclosed. Low concentration of weak acid and weak base are used to respectively prepare spinning solution and coagulating solution of chitosan fibers. The chitosan fibers with diameters below 10 μm are formed in the coagulating solution by rapid stirring. Finally, the coagulating solution containing the chitosan fibers is dried and molded to form fiber matrix.在此揭露幾丁聚醣纖維基材的製備方法。利用低濃度的弱酸及弱鹼來分別配製幾丁聚醣的紡絲液及成型液,並以快速攪拌的方式來形成直徑約10μm以下的幾丁聚醣纖維。最後再讓分佈有幾丁聚醣纖維的成型液進行成型乾燥的步驟,而形成纖維基材。110、120、130、140...步驟