A method for the treatment of fruit plants, in particular Vitis Vinifera, with the use of a sound diffusion system consisting of a plurality of sound speakers (3) arranged inside the vineyard and along the rows of vines (1), in order to generate sound waves which in turn produce acoustic stress for obtaining ameliorating effects of a physiological and entomological nature. Thanks to the method of the invention, the fruit plants are subjected to sound frequency stress, in order to obtain ameliorating effects of the plants from a physiological and entomological point of view, also reducing the use of pesticides and consequent phytosanitary treatment.