A method for performing remote tissue assessment of a tissue region includes selecting imaging parameters, obtaining M image datasets of the tissue region when the tissue region is illuminated by a light signal having a unique discrete wavelength, obtaining a reference image dataset of the tissue region without illumination, processing the M+1 image datasets to obtain M marker maps where each marker map corresponds to a different physiological marker, and analyzing at least one of the marker maps to determine whether a physiological condition exists at the tissue region or to monitor the status of an existing physiological condition at the tissue region. In one or more embodiments, the method may be performed by a portable light source unit including a housing configured for use with a mobile device having a camera, or by a system including a light source unit, an optical sensor module, and a mobile device.
