VARIANTS DU FACTEUR DE TRANSCRIPTION CHIMERIQUE AYANT UNE SENSIBILITE ACCRUE A L'INDUCTION DE LIGAND DE MEDICAMENT D'EXPRESSION TRANSGENIQUE DANS DES CELLULES DE MAMMIFERE
SEATTLE CHILDREN'S HOSPITAL (DBA SEATTLE CHILDREN'S RESEARCH INSTITUTE);SEATTLE CHILDRENS HOSPITAL (DBA SEATTLE CHILDRENS RESEARCH INSTITUTE)
发明人:
JENSEN, MICHAEL C.,OOI, TRACY,WEI, JIA
申请号：
CA3045665
公开号：
CA3045665A1
申请日:
2017.12.11
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
Provided herein is a system for inducible expression of a chimeric antigen receptor in cells. The system comprises: a) a first nucleic acid comprising a first promoter inducible by a drug, wherein the first nucleic acid is operably linked to a first polynucleotide that encodes a chimeric antigen receptor, which comprises a ligand binding domain, a second polynucleotide, which encodes a spacer, a third polynucleotide, which encodes a transmembrane domain and a fourth polynucleotide, which encodes an intracellular signaling domain, and b) a second nucleic acid comprising a second promoter that is operably linked to a nucleic acid encoding a transcriptional activator for the first promoter inducible by drug. Methods of making such cells and methods of treatment using these cells are also provided.
