JARRELL KEVIN A,COLJEE VINCENT W,DONAHUE WILLIAM,MIKHEEVA SVETLANA
申请号：
JP20060348660
公开号：
JP2007075128(A)
申请日:
2006.12.25
申请国别(地区):
日本
年份:
2007
代理人:
摘要：
PROBLEM TO BE SOLVED: To provide an improved system for nucleic acid cloning, which allows DNA ligation with minimal reliance on restriction enzyme, provides for efficient ligation, and is generally useful for the ligation of DNAs having a wide variety of chemical structures. SOLUTION: The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The invention further provides methods for directed ligation of product molecules (i.e., for selective ligation of certain molecules within a collection of molecules). The invention also provides methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems (e.g., ribozyme-mediated systems), and also is susceptible to automation. COPYRIGHT: (C)2007,JPO&INPIT
