The present invention relates to the combination of lysobactin with an aminoglycoside antibiotic for use in the treatment of diseases caused by Gram-positive and Gram-negative bacteria in non-human animals. The invention proved to be particularly useful relating to the treatment of mastitis in non-human animals. Particularly bovine mastitis can be well addressed by the present invention.本發明係關於用於治療非人類動物中由革蘭氏陽性及革蘭氏陰性菌所引起之疾病之溶桿菌素與胺基糖苷抗生素之組合。本發明經證實尤其適用於涉及治療在非人類動物中之乳腺炎。尤其牛乳腺炎可由本發明良好地解決。