PURPOSE: A human DCC1(defective in sister chromatid cohesion 1 homolog)-specific monoclonal antibody is provided to diagnose colorectal cancer and to identify DCC1 molecular physiological function. CONSTITUTION: A monoclonal antibody having human DCC1 specificity is prepared by hybridoma cell(deposit number: KCTC 11611BP). A composition for diagnosing colorectal cancer contains the monoclonal antibody. A kit for diagnosing colorectal cancer contains the composition. A protein chip for diagnosing colorectal cancer contains the monoclonal antibody. The monoclonal antibody is arranged on the predetermined site on a substrate and is fixed in high density.본 발명은 인간 DCC1 (defective in sister chromatid cohesion 1 homolog)에 특이적인 단일클론항체, 이를 생산하는 하이브리도마 세포주, 상기 단일클론항체를 포함하는 대장암 진단용 조성물, 상기 조성물을 포함하는 대장암 진단용 키트 및 상기 단일클론항체를 포함하는 대장암 진단용 단백질 칩에 관한 것이다.