Patent No. 597046 Disclosed is a composition comprising an S. aureus alpha-toxin antigen, a S. aureus Type 5 antigen, a S. aureus type 8 antigen, a microbial surface components recognising adhesive matrix molecules (MSCRAMM) protein and a pharmaceutically acceptable carrier, wherein said alpha-toxin antigen contains at least two alterations, relative to wild-type S. aureus alphatoxin, that reduce its toxicity wherein a first alteration is a chemical alteration and a second alterations is a molecular alteration selected from the group consisting of molecular alteration of amino acids Ala1-Val20, Lys110-Tyr148, Pro103-Thr109, Val149-Asp152, His35, His48, His144, His259, Asp24, Lys37, Lys58, Asp100, Ile107, Glu111, Met113, Asp127, Asp128, Gly130, Gly134, Lys147, Gln150, Asp152, Phe153, Lys154, Val169, Asn173, Arg200, Asn214 and Leu219.
