Operation aseptic is substantially possible, operation is easy, with no risk of splashing to the surroundings and aerosol leakage of droplets such as the chemical preparation, to provide a chemical preparation kit. A chemical liquid preparation kit comprising a transfuse fixture and pre-filled syringe, to seal the tip, with a sealing member that can not be desorbed from said tip, said transfer Note instrument, the pre-filled syringe, the gas in the system is characterized in that it comprises a filter disposed between the one-way valve and capable of discharging irreversibly, so as to be adjacent to the communication passage side of the second from the one-way valve only, chemical liquid preparation kit.実質的に無菌的な操作が可能であり、操作が容易な、薬液調製時に飛沫等の液漏れやエアロゾルの周囲への飛散の虞のない、薬液調製用キットを提供すること。プレフィルドシリンジと移注器具を含む薬液調製用キットであって、前記プレフィルドシリンジは、先端部を密閉する、前記先端部より脱離不能な密閉部材を備え、前記移注器具は、系内の気体のみを不可逆的に排出可能な一方向弁と、前記一方向弁より前記第2の連通路側に隣接するように設けられたフィルターとを具備することを特徴とする、薬液調製用キット。