The present invention relates to a head rotational electroporation device which can be manufactured by a small number of parts in a small size since a positive electrode is connected to a rotational shaft connected to a positive (+) power source and a plurality of negative electrodes are connected to a substrate connected to a negative (-) power source, and can stably provide electric currents for electrodes even though a head is rotated by a rotational driving of a driving motor. According to one embodiment of the present invention, the head rotational electroporation device comprises: a head base which has a motor accommodating unit at a center thereof upwardly protruding and a support ring unit having a pogo pin accommodating unit upwardly protruding from an outer side thereof； a driving motor which is accommodated in the motor accommodating unit； a rotational shaft which is rotated and driven by the driving motor and has a positive (+) power source connected thereto； a pogo pin which is accommodated in the pogo pin accommodating unit and has a negative (-) power source connected thereto； a substrate which is rotated and driven by penetrating and being coupled to the rotational shaft and forms a circular plating line having an upper end portion of the pogo pin elastically contacted thereto； a head casing which is coupled to be able to rotate to the head base, is rotated and driven by the substrate inserted into the inside thereof, forms a first through-hole in a center of the top surface thereof and a second through-hole in an outer side thereof； a positive electrode which is inserted into the first through-hole to be coupled to an upper end of the rotational shaft and is exposed to an upper side of the head casing； and a negative electrode which is inserted into the second through-hole to be connected to the circular plating line of the substrate, and is exposed to an upper side of the head casing.본 발명은 (+)전원에 접속되는 회전축에 양전극에 연결되고 (-)전원에 접속되는 기판에 다수의 음전극이 연결되도록 구
