Disclosed is a laparoscopic port perforation and closure device configured such that a perforation device for perforating a laparoscopic port on a patient's body, a trocar for maintaining the port and allowing various surgical tools to be introduced therethrough, and a closure device for closing the port are selectively used in a single trocar assembly. The device includes: a trocar (4) configured such that a handle is coupled to a first end of a sleeve formed in a tubular shape for being introduced into a perforated port; a penetrating tip (37) with an end tip thereof being exposed outside a second end of the sleeve by sequentially penetrating the handle and the sleeve of the trocar, and configured to enter an abdominal cavity by opening a port; and a combined perforation/closure assembly (3) closing the opened port by replacing the penetrating tip (37) with a closure cartridge (36).