The present invention discloses a preparing method of antispetic medical dressing which include, basificating chitosan fibre to form alkali chitosan fibre, etherificating the alkali chitosan fibre with chlroacetic acid to form carboxymethyl chitosan fibre, then opening, netting, needling the resultant carboxymethyl chitosan fibre to form a dressing. Optionally, the antiseptic carboxymethyl chitosan medical dressing can be made by producing chitosan non-woven cloth with non-woven technique, followed by carboxymethyl reaction with the dressing, cutting, packaging, sterilization. The present invention also discloses the use of antiseptic medical dressing prepared by method mentioned above. The present invention can be applied on surgery wound, burn, scald and other chronic wounds etc, by covering wound it can prevent water in body fluid from losing, providing a active humid condition for wound healing, keeping wound from hydrops, putrescence, isolating bacteria infection, and having the effects which can diminish inflammation, stanch, ease pain, and accelerating the wound healing.