The present disclosure relates to the technical field of traditional Chinese medicine technology, especially to a composition, a preparation method thereof and an application in the prevention and treatment of mammary gland diseases. The present disclosure provides a composition consisting of Rehmanniae Radix Praeparata, Lycii Fructus, Polygonati Rhizoma, Radix Codonopsis Pilosulae, Paeoniae Radix Alba, Cyperi Rhizoma (processed with vinegar) and Grape Seed. The composition has functions of improving hyperplasia of mammary gland and/or anti-oxidation. Compared with each control group, the composition provided by the present disclosure has the optimal effects, and the effect has statistical differences, indicating that the components in the composition provided by the present disclosure are reasonably arranged and can play a good synergistic effect. Furthermore, the preparation is safe and has no side effects.
