The invention relates to a fluid connection device and to a continuous automated system and method for taking fluid microsamples using this device.A device (20) according to the invention, which is intended to be connected to a first line (21) via a first opening and includes a second opening through which a second line (28) passes, comprises:a female fluid connector (22) that defines the first opening and has an internal fitting surface (23) terminating in a female end (24) into which this first line opens anda male fluid connector (25) that defines the second opening, which is fitted into the female connector via its external surface (26) and terminates via its male radial end (27) inside the female connector.According to the invention, this second line is formed from a flexible microtube pushed right through the male connector beyond the male end, the free end of the second line pressing in a sealed manner against the female end so as to minimize the dead volume between the first line and the male connector.
