The present disclosure provides isolated monoclonal antibodies that specifically bind to LAG-3 with high affinity, particularly human monoclonal antibodies. Preferably, the antibodies bind human LAG-3. In certain embodiments, the antibodies bind both human and monkey LAG-3 but do not bind mouse LAG-3. The invention provides anti-LAG-3 antibodies that can inhibit the binding of LAG-3 to MHC Class II molecules and that can stimulate antigen-specific T cell responses. Nucleic acid molecules encoding the antibodies of the invention, expression vectors, host cells and methods for expressing the antibodies of the invention are also provided. Immunoconjugates, bispecific molecules and pharmaceutical compositions comprising the antibodies of the invention are also provided. This disclosure also provides methods for detecting LAG-3, as well as methods for treating stimulating immune responses using an anti-LAG-3 antibody of the invention. Combination therapy, in which an anti-LAG-3 antibody is co-administered with at least one additional immunostimulatory antibody, is also provided.本揭露提供以高親和性與LAG-3特異性結合的經分離之單株抗體(特別是人單株抗體)。較佳地,該等抗體結合人LAG-3。在某些較佳體系中,該等抗體結合人及猴之LAG-3,但不結合小鼠之LAG-3。本發明提供抗LAG-3抗體,該等抗體可抑制LAG-3與MHC第II類分子之結合並可刺激抗原特異性T細胞應答。本發明亦提供編碼本發明之抗體的核酸分子、表達載體、宿主細胞及表達本發明之抗體之方法。本發明亦提供免疫共軛物、雙特異性分子、及包含本發明之抗體之醫藥組成物。本揭露亦提供用於檢測LAG-3之方法以及使用本發明之抗LAG-3抗體以治療刺激性免疫反應之方法。本發明亦提供組合治療,其中抗LAG-3抗體係與至少一種另外之免疫刺激性抗體共同給予。
