The invention relates to the fields of immunology and gene engineering, in particular to construction, preparation and application of a foot-and-mouth disease resistant recombinant vaccine. The vaccine comprises a foot-and-mouth disease virus, in particular a tandem repeat of a VP1 protein epitope of O-type foot-and-mouth disease virus, an immunoglobulin heavy chain constant region or a functional fragment thereof, and a 3D protein of the foot-and-mouth disease virus or an immunogenic fragment thereof. The vaccine can induce animals to generate a protective immunologic response resistant to the foot-and-mouth disease virus.
