METHOD FOR PREPARING TRANSGENIC PLANT WITH INCREASED 20-HYDROXYECDYSONE CONTENT BY USING SPINACIA OLERACEA-DERIVED CYP85 GENE, AND PLANT PREPARED THEREBY
The present invention relates to a method for producing a transgenic plant which has increased content of 20-hydroxyecdysone compared to a wild type plant by transforming a plant cell with a recombinant vector containing a gene encoding CYP85 (cytochrome P450, 85 family) protein derived from spinach (Spinacia oleracea), a transgenic plant with increased content of 20-hydroxyecdysone compared to a wild type plant which is produced by the above method and a seed thereof, a composition for increasing content of 20-hydroxyecdysone in plant which contains a gene encoding CYP85 protein derived from Spinacia oleracea as an effective component, a method for producing a transgenic plant with enhanced insect resistance according to transformation of a plant cell with a recombinant vector containing a gene encoding CYP85 derived from Spinacia oleracea, a transgenic plant with enhanced insect resistance which is produced by the above method and a seed thereof, and a composition for enhancing insect resistance of a plant which contains a gene encoding CYP85 protein derived from Spinacia oleracea as an effective component.
