A nighttime fishing assembly includes a stand that may be positioned on a support surface thereby facilitating the stand to support at least one fishing pole. The stand may be positioned adjacent to a body of water thereby facilitating a line of the fishing pole to be cast into the body of water. A lighting unit is removably coupled to the stand when the stand is positioned in the support surface. The lighting unit emits ultraviolet light thereby facilitating the line from the at least one fishing pole to fluoresce at night.