The invention relates to the field of cell stimulation by mechanical energy, in particular by acoustic energy, for example stimulation by ultrasound, such as focused ultrasound (FUS). It relates to a method for determining a subject specific set of treatment parameters for acoustic wave stimulation and to a method for validating a set of treatment parameters for acoustic wave stimulation. The method for determining a subject specific set of treatment parameters comprises the steps of generating subject specific data, wherein the step of generating subject specific data comprises measuring a geometric property of a body portion, and of determining a target field distribution in the body portion. It comprises further the step of selecting a set of treatment parameters from at least two different sets of treatment parameters, wherein the selection is made prior to a step of applying any set of treatment parameters to the body portion. The method for validating a set of treatment parameters for acoustic wave stimulation comprises the steps of determining a target field distribution of an acoustic field in a body portion, of receiving a related set of treatment parameters for at least one transducer, of generating a subject specific 3D model of the body portion, and of determining a difference between the target field distribution and a field distribution determined in the subject specific 3D model.L'invention se rapporte au domaine de la stimulation cellulaire par énergie mécanique, en particulier par énergie acoustique, par exemple de la stimulation par ultrasons, tels que des ultrasons focalisés (FUS). L'invention concerne un procédé de détermination d'un ensemble de paramètres de traitement spécifique à un sujet permettant une stimulation d'onde acoustique et un procédé de validation d'un ensemble de paramètres de traitement permettant une stimulation d'onde acoustique. Le procédé de détermination d'un ensemble de paramètres de traitement spécifique à un sujet compre
