#$%^&*AU2020101701A420200924.pdf#####CP0017-01-0123 ABSTRACT OF THE DISCLOSURE The application belongs to the field of plant tissue culture, in particular to a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, a preparation method and an application thereof. The culture medium comprises the following components: basic culture medium, plant growth regulator, 95 to 105g/L potato, 4.0 to 5.0g/L agar, 25 to 35g/L sucrose, pH is 5.8 to 6.0. In the present invention, ZT is used as Plant Growth Regulator, which can transport nutrients such as amino acids, auxins, and inorganic salts to the treatment site, which is beneficial to stimulate the growth of buds; The potato contains starch, protein, fat, crude fiber, various microorganisms and inorganic salts, etc., which not only provides various nutrients required for the growth of clump buds, but also facilitates the further elongation of the clump buds. Compared with the existing culture medium, the culture medium of the invention can obviously induce the elongation of clump buds of Vaccinium bracteatum Thunb, effectively shorten the seedling raising period, which is of great significance for the intensive production of the tissue culture of the Vaccinium bracteatum Thunb. (FIG. 1)CP0017-01-0123 FIG.1 FIG.2 1