PROBLEM TO BE SOLVED: To provide an operational method of a manufacturing apparatus of refined water for medical treatment, capable of hot water sterilization.SOLUTION: RO water in an RO water tank is circulated in a circulation line including an active carbon unit 3 and an RO unit 5 for sterilization, while being heated up to higher temperature with a heater 6a. After sterilization, the RO water in the RO water tank 6 is circulated in the circulation line for cooling, while being cooled to lower temperature with a heat exchanger 8.COPYRIGHT: (C)2014,JPO&INPIT【課題】熱水殺菌できる医療用精製水の製造装置の運転方法の提供。【解決手段】RO水タンク6内のRO水をヒータ6aで加熱昇温させながら、活性炭装置3とRO装置5を含む循環ラインに循環させて殺菌する。殺菌後、RO水タンク6内のRO水を熱交換器8で冷却降温させながら、前記循環ラインに循環させて冷却する。【選択図】図1