A puncture apparatus and a puncturing method are disclosed by which a biological tissue is punctured. The puncture apparatus can include an elongated curveable puncture needle which punctures a biological tissue, and a curvature-changing unit which changes curvature of the puncture needle in a process of puncturing the biological tissue by the puncture needle. The puncturing method can include puncturing in such a manner that curvature of the puncture hole varies along a longitudinal direction of the puncture hole, in a process of puncturing the biological tissue.