The present invention relates to transcriptional gene silencing (TGS) of endogenes in plants, plant tissue and plant cells. More specifically, the present invention relates to nucleic acid constructs that are capable of more effectively silencing genes of interest, such as endogenes, in plants, plant tissue and plant cells by TGS. The present invention further relates to methods of more effectively reducing endogenous gene expression in plants, plant tissues or plant cells by TGS using the nucleic acid constructs of the invention.