A bee venom composition with effects of protecting and beautifying a lip comprises a cosmetic matrix component for use on a lip and comprises bee venom. The bee venom is prepared by using the following method: taking out raw bee venom, dissolving the raw bee venom by using water, performing ultra filtration by using an ultra filtration membrane with the molecular weight cutoff 10000Da, and freezing and drying the filter liquor, so as to obtain the bee venom.