Disclosed herein is a use of Fe3−δO4nanoparticle in inhibiting the spore germination of Clostridium difficile . The Fe3−δO4nanoparticle specifically targets the spores of Clostridium difficile spores by damaging and breaking the intact structure of the Clostridium difficile spores. Further, the Fe3−δO4nanoparticle possesses superior sporicidal activity, as compared to other known bactericidal nanoparticles. Therefore, the Fe3−δO4nanoparticle of the disclosure is useful as a bactericide or more specifically, a sporicide to treat Clostridium difficile infection for its superior sporicidal activity.本揭示內容是關於Fe3−δO4奈米粒子於抑制困難梭狀芽孢桿菌之孢子發芽的用途。Fe3−δO4奈米粒子可專一標的至困難梭狀芽孢桿菌孢子，並破壞其結構。相較於其他已知具有殺菌功效的奈米粒子，Fe3−δO4奈米粒子具有更為顯著的殺孢子活性。因此，本揭示內容之Fe3−δO4奈米粒子可用以作為一種殺菌劑，或者更具體來說，一種用以治療困難梭狀芽孢桿菌感染之殺孢子劑。
