Dissociating the cells and tissues of the animal, the step of incubating were seeded in culture vessels with no surface treatment of the cells, and cells comprising the step of selecting the cells attached to the container, method for isolating stem cells.動物由来の組織を細胞に解離させる工程、該細胞を表面処理されていない培養容器に播種してインキュベートする工程、および容器に付着した細胞を選択する工程を含む細胞、特に幹細胞の単離方法。