PURPOSE: A novel Bacillus subtilis CBS and probiotics containing the same are provided to ensure thermal stability, acid resistance and enzyme productivity. CONSTITUTION: A Bacillus subtilis CBS(deposit number KACC91484P) is isolated from straws in a cowhouse. The CBS strain grows at 25°;C-65°;C and pH 6.5-pH 7.5. Probiotics contain the strain, culture medium thereof, or culture extract as an active ingredient. The CBS strain has excellent productivity in the culture medium.본 발명은 신규 바실러스 서브틸리스 CBS(Bacillus subtilis CBS) 균주 및 상기 균주를 포함하는 생균제에 관한 것이다.