The invention relates to a device for determining biological, chemical and/or physical parameters in living biological tissue, comprising an energy supply unit, a laser operating unit with at least one laser source directed at the biological tissue, at least one sensor unit for detecting the light scattered back and/or absorbed by the biological tissue, a control unit, a storing and processing unit and an interface for an external data processing unit. The method according to the invention includes execution of a calibrating phase for ascertaining a reference set (R) of reference vectors (Ri), in each case involving independently ascertaining a parameter (BZi), radiating unpolarized laser light onto the biological tissue and registering a measured value vector (Mi) from a series of optical measured variables and executing an interpolation phase for ascertaining an interpolation set (I) of interpolation vectors (Ik), in each case involving radiating unpolarized laser light onto the biological tissue and registering a measured value vector (Mk) from a back-scattered light intensity with a subsequent determination of an interpolated parameter (BKk) from the reference set (R).
