Provided is a method for inhibiting, effectively and in a stable manner, the translation of mRNA into protein. Disclosed are: a target mRNA translation inhibitor that contains a first polynucleotide which may be hybridized to the 5'-untranslated region and/or translated region of target mRNA, and a second polynucleotide which can be hybridized to the 3'-untranslated region of said mRNA； and/or a method for inhibiting the translation from mRNA using said translation inhibitor. According to the present invention, it is possible to inhibit the translation from target mRNA in a stable manner.提供一種效率佳且安定地抑制自mRNA轉譯至蛋白質的方法。揭示一種標的mRNA之轉譯抑制劑及/或使用前述轉譯抑制劑而抑制自標的mRNA之轉譯的方法，該標的mRNA之轉譯抑制劑包含可與標的mRNA之5’-非轉譯區域及/或轉譯區域雜交的第一多核苷酸、及可與該mRNA之3’-非轉譯區域雜交的第二多核苷酸。依據本發明，可安定地抑制自標的mRNA之轉譯。
