Provided is a process for the production of a protein concentrate from a canola seed and/or rapeseed meal, comprising: i) mixing the meal with a first mixing solvent to form a mixture； ii) optionally adjusting the pH of the mixture to a pH of about 2.0 to about 10.0； iii) separating fiber from the mixture by centrifugation to form a protein slurry and a fiber fraction, wherein the protein slurry comprises: a) a soluble protein fraction； and b) an insoluble protein fraction； wherein the protein is substantially in the protein slurry and the fiber is substantially in the fiber fraction； and the mixture is centrifuged at a speed of about 1,000 - 2,000 rpm； iv) optionally repeating steps i)-iii) by mixing the protein slurry with additional meal； v) optionally mixing the fiber fraction with a second mixing solvent and repeating steps ii) and iii)； v) mixing the protein slurry with an alcohol containing extraction solvent to form an extract and a washed insoluble protein fraction, said washed insoluble fraction comprising the insoluble and soluble protein from the slurry, the soluble protein being denatured or rendered insoluble by contact with the alcohol； vi) separating the extract from the washed insoluble protein fraction； vii) optionally repeating steps v) and vi) at least once； and viii) drying the washed insoluble protein fraction to form a protein concentrate comprising substantially all of the protein from the protein slurry.
