The present invention relates to an isolated limbal stem or progenitor cell comprising a chemically synthesized, recombinant, or isolated nucleic acid that encodes PAX6 that remains chromosomally integrated or non-integrated extrachromosomal genetic material. (LSC) population or LSC-like population, wherein the isolated LSC population is substantially free of non-LSC cells, or the LSC-like population is substantially free of non-LSC-like cells or isolated An LSC or LSC-like population provides an LSC population or LSC-like population, and uses thereof that are substantially free of non-LSC cells and non-LSC-like cells. [Selection] Figure 9e本発明は、染色体に組み込まれた、あるいは組み込まれていない染色体外遺伝物質のままのPAX6をコードする、化学合成、組換え、又は単離核酸を含む、単離された輪部幹若しくは前駆細胞(LSC)集団又はLSC様集団であって、単離されたLSC集団は非LSC細胞を実質的に含まず、又はLSC様集団は非LSC様細胞を実質的に含まず、又は単離されたLSC又はLSC様集団は、非LSC細胞及び非LSC様細胞を実質的に含まない、LSC集団又はLSC様集団、及びそれらの使用を提供する。【選択図】図９ｅ
