Foster, Keith,Chaddock, John,Marks, Philip,Stancombe, Patrick,Aoki, K. Roger,Francis, Joseph,Steward, Lance
申请号：
AU2012201491
公开号：
AU2012201491B2
申请日:
2012.03.13
申请国别(地区):
AU
年份:
2015
代理人:
摘要：
#$%^&*AU2012201491B220150205.pdf#####ABSTRACT A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, 5 or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a nociceptive sensory afferent a Targeting Moiety that is capable of binding to a Binding Site on the nociceptive sensory afferent, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the nociceptive sensory afferent a 10 protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the 15 nociceptive sensory afferent wherein the Targeting Moiety is selected from the group consisting of BAM, p-endorphin, bradykinin, substance P, dynorphin and/or nociceptin. Nucleic acid sequences encoding the polypeptide fusion proteins, methods of preparing same and uses thereof are also described.
