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PROCEDURE FOR CROP PLANTS.
专利权人:
SYNGENTA PARTICIPATIONS AG
发明人:
LAINE, JEAN-MARC,DEVYS, FREDERIC
申请号:
ES05749705
公开号:
ES2375066T3
申请日:
2005.06.14
申请国别(地区):
ES
年份:
2012
代理人:
摘要:
A process for the production of seedlings and / or a reserve of micro-parental plants belonging to the group of herbaceous ornamentals, said procedure comprising an in vitro culture phase during which the explants obtained from the parental reserve of species subject to propagation, are subjected to micro-propagation that is carried out under appropriate conditions and in an appropriate culture medium, in order to produce micro-seedlings that, when subjected to a culture phase in vivo, are intended to develop to give rise to plants or to give rise to the micro-parental reserve, which is characterized in that, to carry out said micro-propagation: a) stem explant stems are obtained from the parental reserve of species subject to propagation, b) the explants are placed, in axenic conditions, in darkness for an appropriate period of time, in a means of initiation of escape that is constituted in such a way that ad suitable for each species of plant being propagated, giving rise to the induction of a first generation of white filament formation, said first generation of filaments comprising one or more auxiliary shoots, c) each white filament is divided into a plurality of pieces, each comprising at least one auxiliary bud, and said filaments and / or pieces obtained from a first generation of white filaments are placed again in axenic conditions and in darkness in a proliferation medium for an appropriate period of time to allow the tissues of the plant cuttings to multiply in order to produce a large number of white filament filaments, comprising between 2 and 10 auxiliary knots, in order to induce the formation of a new generation of white filaments, and the procedure is repeated as much as necessary to produce conse different cuttings of white filaments until the desired amount of white filaments has been obtained; d) and said filaments and / or pieces are placed in axenic conditions, in the presence of light, and for an appropriate period of time, or inside a rootin
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