Sylvain V. Costes,Rafael Gomez-Sjoberg,Steven M. Yannone
申请号：
US14378617
公开号：
US20150017092A1
申请日:
2013.03.14
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Systems and methods for determining the sensitivity of cells (and/or a subject) to ionizing radiation are provided. The systems can comprise a microfluidic device comprising a plurality of microfluidic cavities each configured to contain cells； a source of ionizing radiation configured to deliver ionizing radiation to cells in the microfluidic cavities； and an imaging system configured to detect radiation-induced foci in cells when they are disposed in the microfluidic cavities. The methods can involve contacting a biological sample comprising cells from a subject with ionizing radiation； detecting and quantifying radiation induced foci in the cells at least two different time points； and determining a repair kinetic for radiation induced foci that is a measure of the rate of disappearance of the foci. Methodologies are also provided for in-home blood collection and fixation of nucleated blood cells in a manner to preserve health and fitness biomarkers inherent to these cells.
