The subject invention provides a surgical instrument support assembly which includes an enclosure defining an opening; a light transmissive pane located at least partially across the opening and defining a support surface; and, a light source located inside the enclosure for generating light to be transmitted through the light transmissive pane. A sterilizable light transmissive surgical instrument support may be also provided to be disposed over at least a portion of the light transmissive pane. Advantageously, with the subject invention, an assembly is provided which includes a sterilized surface for supporting surgical instruments which is backlit. With this arrangement, surgical instruments may be supported and illuminated from beneath to aid a medical practitioner in identifying the surgical instruments.