Disclosed are a deodorizer using an autotrophic microbe, and a method for preparing the same. The deodorizer is prepared through a culturing step for amplifying an autotrophic microbe of interest, a sterilizing and purifying step for removing impurities and harmful microbes other than the autotrophic microbe, and a harvesting step for collecting the filtered autotrophic microbes. The microbial deodorizes is effective for removing odorous emissions attributed to ammonia, trimethylamine, formaldehyde, and hydrogen sulfide and is safe to the body and the environment including air and water.
