A fungicidal composition comprising active components A and B, wherein the active component A is benzisothiazolinone, and the active component B is flutriafol； and the weight ratio of the two components is 1:30-30:1. Also provided are a preparation method and use of the composition. Test results show that the fungicidal composition provided in the present invention has an obvious synergistic effect, and more importantly, the application rate is reduced and the cost is reduced. The fungicidal composition is effective in controlling certain specific fungal diseases of the crops. Through the combination of different fungicides with different mechanisms and modes of action, the application rate of each single agent is effectively reduced, and a good effect on broadening the fungicidal spectrum, retarding the fungal resistance and improving the control effect is exhibited.
