#$%^&*AU2013242844A120140501.pdf#####ABSTRACT The present disclosure relates to methods for producing, including intra-operatively obtaining, a fixation device, containing an autologous cell concentrate from a physiological solution, wherein the cell concentrate is isolated without the need for centrifugation, and fixation devices produced thereby.WHOLE BLOOD + SOLUTION A 26 WHOLE BLOOD + SOLUTION A OR BONE MARROW ASPIRIATE 25 31b SUTURE LOADING 31a 28 30 BACKFLUSH SYRINGE SOLUTION B 32b WASTE RESERVOIR FIG. 4A L27 3 I FIG. 4B