ФАН Ин (US),СНЕЙДЕР Эрик Йохн (NL),ФИРТ Эндрю Е. (GB),ЭТКИНС Джон Ф. (IE),ТРЕФФЕРС Эмма Элизабет (NL),ТАС Али (NL),ЛИ Яньхуа (US)
申请号：
RU2015105173
公开号：
RU2015105173A
申请日:
2013.07.18
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. An arterivirus containing a nucleic acid encoding ORF1a or a functional part thereof, wherein said nucleic acid contains at least one mutation resulting in a down translation of the TF domain nsp2TF and / or altered translation of one or more underlying products translated from said nucleic acid into a cell infected with said arterivirus, compared to the wild type of said arterivirus. 2. The arterivirus according to claim 1, wherein said mutation prevents the ribosomal shift of the reading frame -2 at the plot of the reading frame -2 located in the nucleic acid sequence encoding the nsp2.3 protein. The arterivirus according to claim 1 or 2, wherein at least one of said mutations is located in the reading frame shift region of GGU (U / C) U (U / C) U and / or in the conservative CCCANCUCC motif. 4. The arterivirus according to claim 1, wherein at least one of said mutations is in the nucleic acid sequence encoding the nsp1β protein and leads to a reduced ribosomal shift of the reading frame of -2.5. An arterivirus in which nsp2TF function is reduced and / or absent in a cell infected with said arterivirus as compared to the wild type of said arterivirus. 6. The arterivirus according to claim 5, comprising a nucleic acid encoding ORF1 or a functional part thereof, wherein the amino acid sequence nsp2TF of the arterivirus is altered, truncated or absent. The arterivirus according to claim 5 or 6, where the amino acid sequence of the TF domain nsp2TF is modified, and where the specified modified amino acid sequence optionally provides epitopes that are absent in the wild-type arterivirus and are immunogenic, and where the frame1. Артеривирус, содержащий нуклеиновую кислоту, кодирующую ORF1a или его функциональную часть, где указанная нуклеиновая кислота содержит по меньшей мере одну мутацию, приводящую к пониженной трансляции домена TF nsp2TF и/или измененной трансляции одного или более нижележащих продуктов, транслируемых с указанной нуклеиновой кислоты в клетке, ин
