Erik SALLOMONS,Maarten Hotse WILBRINK,Peter SANDERS,Johannis Paulus KAMERLING,Catherina Anna van VUURE,Johannes Adrianus HAGE
申请号：
US15367335
公开号：
US20170080002A1
申请日:
2016.12.02
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The invention relates to a method for providing analogs of human milk oligosaccharides (HMO), in particular oligosaccharides containing terminal sialic acid. The method comprises the steps of: a) providing a source of non-digestible galactooligosaccharides (GOS) containing at least two terminally bonded β-linked galactose residues； b) providing a sialic acid donor having (α2-3)-sialylated O-glycans； c) contacting said GOS with said sialic acid donor in the presence of an enzyme having trans-sialidase activity in an enzyme reaction mixture； and d) isolating from said enzyme reaction mixture a fraction comprising at least 20 percent by weight of disialylated galactooligosaccharides (di-Sia-GOS) based on the dry matter.
