Flush preservation solution for the preservation of cells in the absence of a blood supply comprising:v) water for injection； andvi) at least one saccharide such as a monosaccharide, disaccharide, trisaccharide, or polysaccharide andvii) at least one component with pH buffer properties； andviii) at least one component with calcium transport blocking properties or an anti-calcium action activity；method for the preparation thereof； use thereof in transplantation including organs from heart beating or non heart beating donors, in surgery including any situation of warm or cold ischaemia, cardioplegia or open heart surgery, whole limb or whole body preservation in experimentation on living tissues or in culturing and preserving engineered cells, tissues and organs, limbs or the whole body； method for flushing, preserving or flush preservation of cells； and a kit of parts comprising the solution components.
