A biosafety containment chamber comprises a rigid frame that supports an enclosure made of a flexible, substantially transparent material, which includes at least top and side surfaces joined to provide a fully enclosed environment. A plurality of glove ports are disposed on the enclosure, each including a flexible glove that enables manipulation of items within the enclosure. The biosafety containment chamber further comprises an air supply system to draw air into the fully enclosed environment through an intake HEPA filter, and an air exhaust system to draw air from the fully enclosed environment into an external environment through an exhaust HEPA filter. The air supply system and the air exhaust system are configurable to maintain a negative pressure in the fully enclosed environment.
