A semen storage container which can store semen in a favorable state, into which semen is easily put and out of which semen is easily taken, is provided. A semen storage container 10 includes an outer case 20 with bottom in a cylindrical shape having an open upper end, an inner case 30 with bottom, the inner case having an opening portion 323 at an upper end and being provided on an inner side of the outer case 20 to close an opening at the upper end of the outer case 20, and a removable lid 40 laid over an upper end portion of the outer case 20 to close the opening portion 323 at the upper end of the inner case 30. The inner case 30 includes a semen guide 33 which communicates with the opening portion 323 at the upper end and has an inner circumferential surface provided as an inclined surface 333 which spreads upward and a cylindrical semen pool 34 which communicates with an opening portion 331 at a lower end of the semen guide 33 and includes a tapered bottom portion 342.