The present invention relates to a blood separation container for extracting self-platelet.According to the present invention, since a second coupling portion is formed at a release prevention member coupled to a lower portion of a main body in a PRP separation container, and an ascending member having a bolt structure is screwed to the second coupling portion to ascend a lower cover disposed in a lower fluid chamber of the main body, a separate second main body is unnecessary unlike the related art. Therefore, the PRP separation container is easy to carry, manufacturing costs can be reduced to thereby ensure competitive price, and a PRP can be easily separated and extracted.
