The present invention relates to a starter culture medium for cultivating Lentinus edodes. The starter culture medium is formed by mixing sawdust of an oak tree, fermented sawdust of an oak tree, rice bran, wheat bran, lime, and sugar with water containing germanium to make an optimized cultivation environment in which Lentinus edodes is germinated and grown. The starter culture medium is used for cultivating Lentinus edodes. Due to germanium contained in the starter culture medium, Lentinus edodes, inoculated into the starter culture medium, can be massively produced without diseases.본 발명은 참나무톱밥, 참나무발효톱밥, 미강, 소맥피, 석회, 설탕과, 게르마늄이 함유된 물가 혼합되어 종균배양배지로 참송이종균이 발아되어 성장되도록 촤적의 재배환경을 갖추도록 성형되어 참송이버섯재배용 종균배양배지로 사용되어 종균배양배지에 게르마늄성분으로 인해, 종균배양배지에 접종되는 참송이버섯종균이 질병없이 다량으로 양산하도록 한 참송이버섯재배용 종균배양배지에 관한 것이다.