The present disclosure is directed to systems and methods for delivery of nitric oxide (NO). The methods and systems disclosed herein may provide advantages by controlling NO generation until desired by an end user. To achieve controlled NO generation, embodiments of the disclosure can include multiple compositions that may be in the form of a kit or a multi-chamber delivery system. Additionally, the disclosure contemplates the use of these systems as methods for generating NO by mixing the multiple compositions to yield the controlled generation and/or release of NO. Methods of the disclosure can be used for the controlled delivery of NO to a biological surface (e.g., the skin) and may be used to treat a condition or disease.
