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EFFICIENT PRODUCTION METHOD OF OFFSPRING USING IMPROVED CRYOPRESERVATION OF BOVINE BLASTOCYSTS DERIVED FROM IN VIVO AND IN VITRO FERTILIZATION
专利权人:
DAEGU UNIVERSITY;INDUSTRY ACADEMIC COOPERATION FOUNDATION, DAEGU UNIVERSITY;대구대학교 산학협력단;INDUSTRY ACADEMIC COOPERATION FOUNDATION
发明人:
KOO, DEOG BON,구덕본,MIN, SUNG HUN,민성훈,KOO, DEOG BONKR,MIN, SUNG HUNKR
申请号:
KR1020140107973
公开号:
KR1020160022167A
申请日:
2014.08.19
申请国别(地区):
KR
年份:
2016
代理人:
摘要:
Disclosed is a method for enhancing viability and hatchabilty of blastocyst using in vivo and in vitro-derived blastocyst of Korean native cattle after going through freezing and melting process by means of a slow freezing method. In addition, disclosed is a method for directly transplanting a frozen fertilized egg obtained by the method into a recipient. The selection of in vivo and in vitro-derived blastocyst of Korean native cattle with improved quality is possible by freezing and melting the same after artificially contracting a blastocoelic cavity before the slow freezing. In addition, by directly transplanting the fertilized egg obtained by the method into the recipient, it is possible to remarkably increase productivity of the offspring.본 발명은 한우 생산된 체내·체외 배반포를 이용하여 완만 동결 방법으로 동결 및 융해 후 배반포 생존율 및 부화율 증진방법과 이를 이용하여 수득한 동결수정란을 수란우에 직접 이식하는 방법을 개시하며 완만 동결 전 배반포강을 인위적으로 수축시켜 동결 및 융해 후 질적으로 개선된 한우 체내 및 체외 배반포의 선발과 이를 이용하여 수득한 수정란을 수란부에 직접 이식함으로써 산자 생산 효율을 현저히 증진시킬 수 있는 뛰어난 효과가 있다.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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