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How to control the recombination vector, the plant, the amount of mevalonic acid production, and the amount of isoprenoid production which hold the manufacturing method of mutant protein and the mutant protein, the gene which codes the mutant protein, and the gene, the control method of 3-hydroxy 3-methyl glutaryl CoA Reductase activity
专利权人:
OSAKA UNIVERSITY;SUMITOMO RUBBER INDUSTRIES, LTD
发明人:
KAZUHISA FUSHIHARA,KEIJI TAKAGI,KEIKO SUZUKI,ROBERTLEE JEKSON,TOSHIYA MURANAKA,YUKINO INOUE
申请号:
BR112016027261
公开号:
BR112016027261A2
申请日:
2015.05.22
申请国别(地区):
BR
年份:
2018
代理人:
摘要:
A mutant protein obtained by mutating an hmgr-specific amino acid residue, an enzyme limiting the isoprene biosynthesis rate in the polyisoprenoid biosynthesis pathway, is provided. The present invention relates to a mutant protein wherein at least one amino acid residue selected from the group consisting of amino acid residues at positions 91, 225, 257, 287, 339, 411, 470, 509 and 574 of Arabidopsis thaliana 3-hydroxy-3-methylglutaryl coa reductase shown by sequence: 1 and amino acid residues at the positions corresponding to those mentioned above in 3-hydroxy-3-methylglutaryl coa reductase is deleted or replaced by another amino acid.é fornecida uma proteína mutante obtida por mutação de um resíduo de aminoácido específico da hmgr, uma enzima limitante da taxa de biossíntese do monômero isopreno na via de biossíntese poliisoprenoid. a presente invenção refere-se a uma proteína mutante, em que pelo menos um resíduo de aminoácido selecionado a partir do grupo que consiste em resíduos de aminoácidos nas posições 91, 225, 257, 287, 339, 411, 470, 509 e 574 da 3-hidroxi-3-metilglutaril-coa-redutase de arabidopsis thaliana mostrada pela seq id no: 1 e os resíduos de aminoácidos nas posições correspondentes aos acima mencionados no 3-hidroxi-3-metilglutaril coa redutase é deletado ou substituído por outro resíduo de aminoácido.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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