A bone regenerating agent containing stem cells as a raw material and containing an inactivated cell mass and the inactivated cell mass including at least a calcified substance and an extracellular matrix has osteoconductive ability and osteoinductive ability and can be manufactured at low cost, Can be controlled. Also, (1) a step of culturing the stem cells at 0.01 to 1.00 Hz while inducing differentiation into cells which produce calcified products while shaking culturing to obtain cell aggregates containing at least calcified substances and extracellular matrix, or ) Stem cells overexpressing proteins involved in osteogenesis by gene manipulation are induced to differentiate into cells that produce calcified products by shaking culture or static culture, and contain at least calcified substances and extracellular matrix A step of obtaining a cell aggregate, and (2) a step of inactivating the cell aggregate obtained in the above step, the bone regenerating agent can be easily produced.幹細胞を原料とし、不活化細胞塊を含み、前記不活化細胞塊が少なくとも石灰化物及び細胞外基質を含む骨再生剤は、骨伝導能及び骨誘導能を有し、低コストで製造でき、サイズを制御できる。また、(1)幹細胞を0.01~1.00Hzで振盪培養しながら石灰化物を産生する細胞に分化誘導し、少なくとも石灰化物及び細胞外基質を含有する細胞凝集体を得る工程、又は(1’)遺伝子操作によって骨形成に関与するタンパク質を過剰発現させた幹細胞を、振盪培養あるいは静置培養をして、石灰化物を産生する細胞に分化誘導し、少なくとも石灰化物及び細胞外基質を含有する細胞凝集体を得る工程、及び(2)前記工程で得られた細胞凝集体を不活性化処理する工程を有する方法は、上記骨再生剤を簡便に製造できる。
